Cells under jetting flow conditions

Cells being exposed to a fast liquid flows display a rupture of their cell membrane. In this project the interaction of fast liquid jets with cells has been studied within two approaches.
a) A liquid jet flow much larger than the size of the cell (approx. 10 micron) is directed on plated cells adherent on a substrate. As the jet spreads on the surface covered with cells a boundary layer flow with strong shear gradients is set-up.
b) A fast jet flow with a few microns in diameter is pointed to a single, fixed cell to probe the cell membrane stiffness and its yield strength for poration.

Figure 1: Sonoporation by jetting cavitation bubble. Viable porated cells are colored green (calcein uptake) and red cells are stained with ethidium bromide (permanent poration). Image size ~1mm².

Info:Detlef Lohse

Researchers: Manish Arora, Rory Dijkink, Claus-Dieter Ohl, Andrea ProsperettiDetlef Lohse.
Embedding: BMTI, JMBC
Sponsors: NWO


Controlled cavitation–cell interaction: trans-membrane transport and viability studies
R. Dijkink, S. Le Gac, E. Nijhuis, A. van den Berg, I. Vermes, A. Poot, and C.D. Ohl
Phys. Med. Biol. 53, 375 (2007)BibTeΧ
Measurement of cavitation induced wall shear stress
R. Dijkink and C.D. Ohl
Appl. Phys. Lett. 93, 254107 (2008)BibTeΧ
Sonoporation of suspension cells with a single cavitation bubble in a microfluidic confinement
S. Le Gac, E. Zwaan, A. van den Berg, and C.D. Ohl
Lab Chip 7, 1666–1672 (2007)BibTeΧ
Sonoporation from jetting cavitation bubbles
C.D. Ohl, M. Arora, R. Ikink, N. de Jong, M. Versluis, M. Delius, and D. Lohse
Biophys. J. 91, 4285–4295 (2006)BibTeΧ

Max Planck Gesellschaft
4TU Precision Medicine
Centre for Scientific Computing