Physics of Fluids - Publications

The response of dual-species bacterial biofilm to 2% and 5% NaOCl mixed with etidronic acid: A laboratory real-time evaluation using optical coherence tomography

Open Access
International Endodontic Journal 55, 758–771 (2022)

Authors

	M.M.B. Borges
	R.J.B. Dijkstra
	F.B. Andrade
	M.A.H. Duarte
	Michel Versluis
	Lucas W. M. van der Sluis
	X. Petridis

BibTeΧ

@article{https://doi.org/10.1111/iej.13754,
author = {Borges, Mariana Maciel Batista and Dijkstra, Ren√© J. B. and de Andrade, Flaviana Bombarda and Duarte, Marco Antonio Hungaro and Versluis, Michel and van der Sluis, Lucas W. M. and Petridis, Xenos},
title = {The response of dual-species bacterial biofilm to 2\% and 5\% NaOCl mixed with etidronic acid: A laboratory real-time evaluation using optical coherence tomography},
journal = {International Endodontic Journal},
volume = {55},
number = {7},
pages = {758-771},
keywords = {biofilm, etidronate, HEDP, irrigants, optical coherence tomography, sodium hypochlorite},
doi = {https://doi.org/10.1111/iej.13754},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/iej.13754},
eprint = {https://onlinelibrary.wiley.com/doi/pdf/10.1111/iej.13754},
abstract = {Abstract Aim The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2\% and 5\% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow. Methodology Dual-species biofilms comprised of Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were grown on human dentine discs in a constant depth film fermenter (CDFF) for 96¬†h. Biofilms were treated with 2\% and 5\% NaOCl, alone or mixed with HEDP. Irrigants were applied under steady laminar flow for 8¬†min. Biofilm response was evaluated by means of optical coherence tomography (OCT). Biofilm removal, biofilm disruption, rate of biofilm loss and disruption as well as bubble formation were assessed. One-way anova, Wilcoxon's signed-rank test and Kruskal‚ÄìWallis H test were performed for statistical analysis of the data. The level of significance was set at a ‚â§.05. Results Increasing NaOCl concentration resulted in increased biofilm removal and disruption, higher rate of biofilm loss and disruption and increased bubble formation. Mixing HEDP with NaOCl caused a delay in the antibiofilm action of the latter, without compromising its antibiofilm efficacy. Conclusions NaOCl concentration dictates the biofilm response irrespective of the presence of HEDP. The addition of HEDP resulted in a delay in the antibiofilm action of NaOCl. This delay affects the efficiency, but not the efficacy of the irrigant over time.},
year = {2022}
}