Physics of Fluids - Publications

Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound

Journal of Controlled Release 316, 79 – 92 (2019)

Authors

BibTeΧ

@article{ROOVERS201979,
title = "Sonoprinting liposomes on tumor spheroids by microbubbles and ultrasound",
journal = "Journal of Controlled Release",
volume = "316",
pages = "79 - 92",
year = "2019",
issn = "0168-3659",
doi = "https://doi.org/10.1016/j.jconrel.2019.10.051",
url = "http://www.sciencedirect.com/science/article/pii/S0168365919306157",
author = "S. Roovers and J. Deprez and D. Priwitaningrum and G. Lajoinie and N. Rivron and H. Declercq and O. De Wever and E. Stride and S. Le Gac and M. Versluis and J. Prakash and S.C. De Smedt and I. Lentacker",
keywords = "Microbubbles, Ultrasound, Drug delivery, Loaded Microbubbles, Mechanisms, Sonoprinting",
abstract = "Ultrasound-triggered drug-loaded microbubbles have great potential for drug delivery due to their ability to locally release drugs and simultaneously enhance their delivery into the target tissue. We have recently shown that upon applying ultrasound, nanoparticle-loaded microbubbles can deposit nanoparticles onto cells grown in 2D monolayers, through a process that we termed “sonoprinting”. However, the rigid surfaces on which cell monolayers are typically growing might be a source of acoustic reflections and aspherical microbubble oscillations, which can influence microbubble-cell interactions. In the present study, we aim to reveal whether sonoprinting can also occur in more complex and physiologically relevant tissues, by using free-floating 3D tumor spheroids as a tissue model. We show that both monospheroids (consisting of tumor cells alone) and cospheroids (consisting of tumor cells and fibroblasts, which produce an extracellular matrix) can be sonoprinted. Using doxorubicin-liposome-loaded microbubbles, we show that sonoprinting allows to deposit large amounts of doxorubicin-containing liposomes to the outer cell layers of the spheroids, followed by doxorubicin release into the deeper layers of the spheroids, resulting in a significant reduction in cell viability. Sonoprinting may become an attractive approach to deposit drug patches at the surface of tissues, thereby promoting the delivery of drugs into target tissues."
}

	S. Roovers
	Joke Deprez
	D. Priwitaningrum
	Guillaume Lajoinie
	H. Declercq
	O. De Wever
	Eleanor Stride
	Séverine Le Gac
	Michel Versluis
	J. Prakash
	S.C. De Smedt
	Ine Lentacker