Cells under jetting flow conditions
Cells being exposed to a fast liquid flows display a rupture of their cell membrane. In this project the interaction of fast liquid jets with cells has been studied within two approaches.
a) A liquid jet flow much larger than the size of the cell (approx. 10 micron) is directed on plated cells adherent on a substrate. As the jet spreads on the surface covered with cells a boundary layer flow with strong shear gradients is set-up.
b) A fast jet flow with a few microns in diameter is pointed to a single, fixed cell to probe the cell membrane stiffness and its yield strength for poration.
Figure 1: Sonoporation by jetting cavitation bubble. Viable porated cells are colored green (calcein uptake) and red cells are stained with ethidium bromide (permanent poration). Image size ~1mm².