| Mon December 8th 2014 14:30 – 16:00 ZH286 | Master defense | Cell transfection using spark-induced cavitation bubble in narrow gap Anne Charlotte van Blokland |
Details:A transient and strong liquid flow allows cells to take-up foreign molecules into the cells without their detachment. In this study a simple and cheap spark device is designed and tested to generate a spark-induced cavitation bubble in a narrow gap. The gap height between two fixed walls was varied and three bubble collapse scenarios were observed; collapse towards the opposite wall, neutral collapse and collapse towards the upper wall. The uptake of fluorescent dyes (calcein and FITC-Dextran) was observed after single cavitation event. The maximum molecular uptake of the foreign molecules was found at the smallest gap height. | ||
